X-100, 20 mM Tris, 40 mM NaF, 0.2 SDS, 0.five deoxycholate, 1 mM EDTA, 1 mM EGTA, 1 mM Na3 VO4 , one hundred mM NaCl, pH 7.5). The extract was centrifuged at 15,000g for 15 min at four C then stored at -20 C before use. Target protein in liver tissue sample was applied for Western blot based on the earlier research [40,41]. The samples have been separated on 10 SDS-PAGE gels and then transferred to polyvinylidene fluoride membranes. PPAR- polyclonal antibody (sc-398394, Santa Cruz Biotechnology Inc., Dallas, TX, USA), acetyl-CoA carboxylase (ACC) monoclonal antibody (3676, Cell Signaling Technology Inc., Danvers, MA, USA), -actin monoclonal antibody (MA5-15739, Thermo Fisher Scientific, Rockford, IL, USA) had been made use of to establish the protein expression of PPAR-, ACC, and -actin by utilizing immunoblotting. four.9. Statistic Evaluation The data are commonly distributed and expressed because the imply SD (n = 8). Significance between the groups was determined by one-way evaluation of variance (ANOVA) followed by Duncan’ s various range test by using SPSS 12.0. (SPSS Institute, Inc., Chicago, IL, USA). Differences with p 0.05 had been viewed as statistically significant.Author Contributions: T.-A.L.; funding acquisition, S.-C.C. and C.-L.L.; investigation, T.-A.L., S.-C.C. and C.-L.L.; project administration, S.-C.C. and C.-L.L.; resources, S.-C.C.; visualization, C.-L.L.; writing–original draft, T.-A.L., B.-J.K. and C.-L.L.; writing–review and editing, C.-L.L. All authors have read and agreed towards the published version of your manuscript. Funding: This investigation received no external funding. Institutional Evaluation Board Statement: The study was performed according to the recommendations on the Declaration of Helsinki and authorized by the Institutional Animal Care and Use Committee (IACUC) in the National Taitung University (protocol code 100115 of approval). Informed Consent Statement: Not applicable. Data Availability Statement: The data presented in this study are accessible on request from the corresponding author. The information are usually not publicly available on account of ethical restriction. Acknowledgments: We thank Sinfong Agricultural Technologies Enterprise (Taipei, Taiwan) for FP Antagonist drug providing red quinoa. Conflicts of Interest: The authors declare no conflict of interest. Sample Availability: Samples from the red quinoa are accessible from the authors.Molecules 2021, 26,13 of
International Journal ofEnvironmental HDAC5 Inhibitor review Analysis and Public HealthArticleHealth Status Is Impacted, and Phase I/II Biotransformation Activity Altered in Young Girls Making use of Oral Contraceptives Containing Drospirenone/Ethinyl EstradiolGerda Venter , Carien L. van der Berg , Francois H. van der Westhuizen and Elardus Erasmus Human Metabolomics, Faculty of Natural and Agricultural Sciences, North-West University (Potchefstroom Campus), 11 Hoffman Street, Potchefstroom 2531, South Africa; [email protected] (C.L.v.d.B.); [email protected] (F.H.v.d.W.) Correspondence: [email protected] (G.V.); [email protected] (E.E.); Tel.: +27-18-299-1867 (G.V.); +27-18-299-2305 (E.E.)Citation: Venter, G.; van der Berg, C.L.; van der Westhuizen, F.H.; Erasmus, E. Health Status Is Impacted, and Phase I/II Biotransformation Activity Altered in Young Women Utilizing Oral Contraceptives Containing Drospirenone/Ethinyl Estradiol. Int. J. Environ. Res. Public Well being 2021, 18, 10607. doi.org/10.3390/ijerph182010607 Academic Editor: Paul B. Tchounwou Received: 31 August 2021 Accepted: 3 October 2021 Published: 10 Octo
