Name :
Recombinant Mouse PCSK9 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Biological Activity :
Measured by its binding ability in a functional ELISA
. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR
. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
Expression Host :
Mouse
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_705793.1
NCBI Gene ID :
Synonyms :
Synonyms :
proprotein convertase subtilisin/kexin type 9
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant mouse PCSK9 consists of 671 amino acids and has a calculated molecular mass of 72.6 kDa. As a result of proteolysis and glycosylation, the recombinant protein migrates as doublet with apparent molecular mass of approximately 19 & 65 kDa, cprresponding to the prodomain and the mature form of PCSK9 respectively in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the full length of mouse PCSK9 (NP_705793.1) precursor (Met 1-Gln 694) was expressed, with a C-terminal polyhistidine tag.
Buffer Solution :
Supplied as sterile 15mM Tris, 90mM NaCl, 50% Glycerol, pH 7.5Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
AI415265 Protein, Mouse; AI747682 Protein, Mouse; FH3 Protein, Mouse; HCHOLA3 Protein, Mouse; Narc1 Protein, Mouse; PC9 Protein, Mouse PCSK9 背景信息 Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
References & Citations :
Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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