Name :
Recombinant Rat Transferrin Receptor/TFR1/CD71 Protein (His Tag)
Biological Activity :
Background :
Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
Biological Activity :
Testing in progress
Expression Host :
Rat
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q99376
NCBI Gene ID :
Synonyms :
Synonyms :
transferrin receptor
Amino Acid Sequence :
Molecular Weight :
The recombinant Rat TFRC consists of 694 amino acids and predicts a molecular mass of 78.59 kDa. It migrates as an approximately 73.45 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Rat TFRC (Q99376) (Gly87-Phe761) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 150 mM NaCl, 0.02% tween80, 5% Mannitol, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Douabin-Gicquel V., et al., 2001,Hum. Genet. 109:393-401. Ryschich,E. et al., 2004,Eur J Cancer. 40 (9):1418-22. Tosoni D., et al., 2005, Cell 123:875-888. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
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