On, divided by background, defined as initial fluorescence). The improvement was most marked for the higher wavelength dyes. In addition, with an absorption range from 390 nm to 625 nm (Figure 3), the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes. As is normal these days, the use of these products is restricted and users should heed the following qualification statement:
“These Products are for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Products are subject to proprietary rights of Epoch Biosciences, Inc. and are made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to the Products and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of the Products. “Commercial use” includes but is not limited to the sale, lease, license or other transfer of the Products or any material derived or produced from them, the sale, lease, license or other grant of rights to use the Products or any material derived or produced from them, or the use of the Products to perform services for a fee for third parties (including contract research).1160247-92-6 Formula ” Redmond Red, Yakima Yellow, and Eclipse are trademarks of Epoch Biosciences, Inc.
A few months ago, we introduced the new
3′-Amino-Modifier supports, shown in Figure 1, (1) and (2). In this alternative approach1, the nitrogen destined to become the 3′-amino group is included in a phthalimide (PT) group which is attached to the support through an amide group on the aromatic ring. This simple linkage is very stable to all conditions of oligonucleotide synthesis and contains no chiral center.2089288-03-7 manufacturer In the last Glen Report2, we compared yields and purity of crude oligonucleotides produced with 3′-Amino-Modifier C7 CPG (3) and 3′-PT-Amino-Modifier C6 CPG (2).PMID:28613663 The results indicated that the yield of product from the 3′-PT-Amino-Modifier C6 CPG is about 20% lower if deprotected with ammonium hydroxide. However, the purity of amino-modified product is significantly higher due to the absence of the acetyl capped product. One of our main concerns about the PTAmino supports was the efficiency of the cleavage reaction from the support by ammonium hydroxide, which led to the lower yield of crude oligonucleotide. Also, we felt it was necessary to compare the cleavage efficiencies of the PT-AminoModifier supports under a variety of other, commonly used oligonucleotide deprotection conditions, since a wide range of conditions has had to be developed for the deprotection of modified oligonucleotides. For these supports to become universally accepted, they have to be capable of use with the diverse group of modified bases and dyes we offer that has various sensitivities to basic conditions. A series of experiments was set up to examine the products from 3′-PT-AminoModifier C3 and C6 CPG after deprotection by a variety of popular techniques. As an experimental control, the 3′-aminomodified oligonucleotides were compared to a thymidyl 20mer prepared from a standard, ester-linked, thymidine CPG. Results The experiments were carried out by synthesizing a thymidyl 20mer on a 15 umole scale on each of the following supports: 3′-PT-Amino-Modifier C3 and C6 CPG, and T-CPG. The initial dimethoxytrityl solutions were collected so that the exact 4
Support Thymidine (1) Thymidine (2) 3′-PT-Amino-Modifier C3 CPG (1) 3′-PT-Amino-Modifie.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
