Poralla K Squalene-hopene cyclase from Methylococcus capsulatus: a bacterium creating hopanoids and steroids. Biochim Biophys Acta 1391: 223232. 19. Kleemann G, Kellner R, Poralla K Purification and properties of your squalene-hopene cyclase from Rhodopseudomonas palustris, a purple non-sulfur bacterium producing hopanoids and tetrahymanol. Biochim Biophys Acta 1210: 317320. 20. Ochs D, Kaletta C, Entian KD, Beck-Sickinger A, Poralla K Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism. J Bacteriol 174: 298302. 21. Wendt KU, Lenhart A, Schulz GE The structure in the membrane protein squalene-hopene cyclase at 2.0 A resolution. J Mol Biol 286: 175187. 22. Jurgens UJ, Simonin P, Rohmer M Localization and distribution of hopanoids in membrane systems with the cyanobacterium Synechocystis PCC-6714. FEMS Microbiol Lett 92: 285288. 23. Feil C, Sussmuth R, Jung G, Poralla K Site-directed mutagenesis of putative active-site residues in squalene-hopene cyclase. Eur J Biochem 242: 51 55. 24. Wendt KU, Poralla K, Schulz GE Structure and function of a Epigenetics squalene cyclase. Science 277: 18111815. 25. Pearson A, Rusch DB Distribution of microbial terpenoid lipid cyclases in the worldwide ocean metagenome. The ISME journal three: 352363. 26. Rohmer M, Bouviernave P, Ourisson G Distribution of hopanoid triterpenes in prokaryotes. J Gen Microbiol 130: 11371150. 27. Lee S, Poulter CD Cloning, inhibitor solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1. J Bacteriol 190: 3808 3816. 28. Sozer O, Komenda J, Ughy B, Domonkos I, Laczko-Dobos H, et al. Involvement of carotenoids within the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803. Plant Cell Physiol 51: 823835. 29. Gu P, Ishii Y, Spencer TA, Shechter I Function-structure research and identification of three enzyme domains involved in the catalytic activity in rat hepatic squalene synthase. J Biol Chem 273: 1251512525. 30. Summers C, Karst F, Charles AD Cloning, expression and characterisation with the cDNA encoding human hepatic squalene synthase, and its partnership to phytoene synthase. Gene 136: 185192. 31. Bligh EG, Dyer WJ A speedy method of total lipid extraction and purification. Can J Biochem Physiol 37: 911917. 32. Schmerk CL, Bernards MA, Valvano MA Hopanoid production is required for low-pH tolerance, antimicrobial resistance, and motility in Burkholderia cenocepacia. J Bacteriol 193: 67126723. 33. Welander PV, Hunter RC, Zhang L, Sessions AL, Summons RE, et al. Hopanoids play a function in membrane integrity and pH homeostasis in Rhodopseudomonas palustris TIE-1. J Bacteriol 191: 61456156. 34. Seipke RF, Loria R Hopanoids will not be important for development of Streptomyces scabies 87-22. J Bacteriol 191: 52165223. 35. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G Purification and properties of unicellular blue-green algae. Bacteriol Rev 35: 171205. 36. Kufryk GI, Sachet M, Schmetterer G, Vermaas WF Transformation with the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS Microbiol Lett 206: 215219. 37. Huang HH, Camsund D, Lindblad P, Heidorn T Design and style and characterization of molecular tools for any Synthetic Biology approach towards creating cyanobacterial biotechnology. Nucleic Acids Res 38: 25772593. 38. Elhai J, Wolk CP Conjugal transfer of DNA to cyanobacteria. Approaches Enzymol 167: 747754. 39. Gibson DG, Young L,.Poralla K Squalene-hopene cyclase from Methylococcus capsulatus: a bacterium generating hopanoids and steroids. Biochim Biophys Acta 1391: 223232. 19. Kleemann G, Kellner R, Poralla K Purification and properties in the squalene-hopene cyclase from Rhodopseudomonas palustris, a purple non-sulfur bacterium making hopanoids and tetrahymanol. Biochim Biophys Acta 1210: 317320. 20. Ochs D, Kaletta C, Entian KD, Beck-Sickinger A, Poralla K Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism. J Bacteriol 174: 298302. 21. Wendt KU, Lenhart A, Schulz GE The structure of the membrane protein squalene-hopene cyclase at two.0 A resolution. J Mol Biol 286: 175187. 22. Jurgens UJ, Simonin P, Rohmer M Localization and distribution of hopanoids in membrane systems in the cyanobacterium Synechocystis PCC-6714. FEMS Microbiol Lett 92: 285288. 23. Feil C, Sussmuth R, Jung G, Poralla K Site-directed mutagenesis of putative active-site residues in squalene-hopene cyclase. Eur J Biochem 242: 51 55. 24. Wendt KU, Poralla K, Schulz GE Structure and function of a squalene cyclase. Science 277: 18111815. 25. Pearson A, Rusch DB Distribution of microbial terpenoid lipid cyclases within the worldwide ocean metagenome. The ISME journal 3: 352363. 26. Rohmer M, Bouviernave P, Ourisson G Distribution of hopanoid triterpenes in prokaryotes. J Gen Microbiol 130: 11371150. 27. Lee S, Poulter CD Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1. J Bacteriol 190: 3808 3816. 28. Sozer O, Komenda J, Ughy B, Domonkos I, Laczko-Dobos H, et al. Involvement of carotenoids within the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803. Plant Cell Physiol 51: 823835. 29. Gu P, Ishii Y, Spencer TA, Shechter I Function-structure research and identification of 3 enzyme domains involved in the catalytic activity in rat hepatic squalene synthase. J Biol Chem 273: 1251512525. 30. Summers C, Karst F, Charles AD Cloning, expression and characterisation on the cDNA encoding human hepatic squalene synthase, and its relationship to phytoene synthase. Gene 136: 185192. 31. Bligh EG, Dyer WJ A rapid strategy of total lipid extraction and purification. Can J Biochem Physiol 37: 911917. 32. Schmerk CL, Bernards MA, Valvano MA Hopanoid production is essential for low-pH tolerance, antimicrobial resistance, and motility in Burkholderia cenocepacia. J Bacteriol 193: 67126723. 33. Welander PV, Hunter RC, Zhang L, Sessions AL, Summons RE, et al. Hopanoids play a part in membrane integrity and pH homeostasis in Rhodopseudomonas palustris TIE-1. J Bacteriol 191: 61456156. 34. Seipke RF, Loria R Hopanoids will not be critical for growth of Streptomyces scabies 87-22. J Bacteriol 191: 52165223. 35. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G Purification and properties of unicellular blue-green algae. Bacteriol Rev 35: 171205. 36. Kufryk GI, Sachet M, Schmetterer G, Vermaas WF Transformation of your cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS Microbiol Lett 206: 215219. 37. Huang HH, Camsund D, Lindblad P, Heidorn T Design and characterization of molecular tools for any Synthetic Biology approach towards building cyanobacterial biotechnology. Nucleic Acids Res 38: 25772593. 38. Elhai J, Wolk CP Conjugal transfer of DNA to cyanobacteria. Strategies Enzymol 167: 747754. 39. Gibson DG, Young L,.
