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E above), it is impossible to eliminate it completely. To take this limitation into account, we randomly added 0.01 , 0.05 , and 0.1 substitution errors per base to reproduce different levels of noise.Global haplotype reconstructionSimulated reads were used as input to the global haplotype reconstruction procedure of ShoRAH using the programs `contain’, `mm.py’, and `freqEst’. Global haplotype inference was applied here only to the simulated data with a controlled sequencing error rate and hence ShoRAH was run without error correction. We considered the reads that are compatible with each other, i.e., that are identical on an overlapping region, and built the read graph, whose vertices correspond to reads and edges connect compatible reads. Haplotypes were reconstructed as paths in the read graph, such that all reads are explained by a minimal number of haplotypes. The relative frequencies of all inferred haplotypes 1326631 are then estimated using an Expectation Maximization algorithm [2,17].ResultsFigure 1. Diversity of the protease region measured on the multiple sequence alignments. The plot shows the get AN-3199 Shannon entropy of each column of the multiple sequence alignment of allWe prepared a genetically diverse DNA sample by mixing ten HIV clones isolated from infected patients. One aliquot of this mixture was subject to PCR amplification. These two samples were sequenced in parallel using 454/Roche and IlluminaViral Quasispecies ReconstructionTable 2. Performance of local haplotype reconstruction.Platform 454/Roche 454/Roche Illumina GA Illumina GAPCR amplification No Yes No YesReconstructed 13 30 10TP 5 6 9FP 8 24 1FN 5 4 1Sensitivity [ ] 50 60 90Specificity [ ] 38 20 90For all four experiments, we report the total number of predicted haplotypes (column Reconstructed), the number of correct haplotypes (true positives, TP), the number of reconstructed haplotypes that do not match any of the original clones (false positives, FP), and the number of missed haplotypes (false negatives, FN). This number is equal to 10 ?TP, because ten is the total number of haplotypes present in the sample. Sensitivity is 1418741-86-2 biological activity defined as TP/(TP+FN) and specificity as TP/(TP+FP). Local haplotype reconstruction was performed on the 252 bp region of the HIV pol gene coding for protease amino acids 10 to 93 for the 454/Roche data, and on the 35 bp subregion of highest entropy for the Illumina reads. doi:10.1371/journal.pone.0047046.tGenome Analyzer, yielding a total of four experiments (Table 1). A total of 668 and 4,331 reads from 454/Roche sequencing were analyzed for the non-PCR amplified and PCR amplified sample, respectively. These numbers include all reads overlapping at least 80 of the amino acids 10 to 93 of the HIV-1 protease and represent the coverage of this region, which hosts the mutations associated with resistance to protease inhibitors. Segments of the reads falling outside of this region were discarded. The length of the remaining segments is 232616 bases (mean 6 std) and 236618 bases for the two 454/Roche samples. Since we are dealing with a coding region, all insertions causing a frameshift were discarded. We did not detect any amino acid insertion or deletion. The Illumina experiments had a much higher throughput with more than one million reads mapped to the protease and local coverage of around 10,000 reads per base pair in the region further analyzed (Table 1). Reads from the 454/Roche platform are long enough to display the diversity of the viral pop.E above), it is impossible to eliminate it completely. To take this limitation into account, we randomly added 0.01 , 0.05 , and 0.1 substitution errors per base to reproduce different levels of noise.Global haplotype reconstructionSimulated reads were used as input to the global haplotype reconstruction procedure of ShoRAH using the programs `contain’, `mm.py’, and `freqEst’. Global haplotype inference was applied here only to the simulated data with a controlled sequencing error rate and hence ShoRAH was run without error correction. We considered the reads that are compatible with each other, i.e., that are identical on an overlapping region, and built the read graph, whose vertices correspond to reads and edges connect compatible reads. Haplotypes were reconstructed as paths in the read graph, such that all reads are explained by a minimal number of haplotypes. The relative frequencies of all inferred haplotypes 1326631 are then estimated using an Expectation Maximization algorithm [2,17].ResultsFigure 1. Diversity of the protease region measured on the multiple sequence alignments. The plot shows the Shannon entropy of each column of the multiple sequence alignment of allWe prepared a genetically diverse DNA sample by mixing ten HIV clones isolated from infected patients. One aliquot of this mixture was subject to PCR amplification. These two samples were sequenced in parallel using 454/Roche and IlluminaViral Quasispecies ReconstructionTable 2. Performance of local haplotype reconstruction.Platform 454/Roche 454/Roche Illumina GA Illumina GAPCR amplification No Yes No YesReconstructed 13 30 10TP 5 6 9FP 8 24 1FN 5 4 1Sensitivity [ ] 50 60 90Specificity [ ] 38 20 90For all four experiments, we report the total number of predicted haplotypes (column Reconstructed), the number of correct haplotypes (true positives, TP), the number of reconstructed haplotypes that do not match any of the original clones (false positives, FP), and the number of missed haplotypes (false negatives, FN). This number is equal to 10 ?TP, because ten is the total number of haplotypes present in the sample. Sensitivity is defined as TP/(TP+FN) and specificity as TP/(TP+FP). Local haplotype reconstruction was performed on the 252 bp region of the HIV pol gene coding for protease amino acids 10 to 93 for the 454/Roche data, and on the 35 bp subregion of highest entropy for the Illumina reads. doi:10.1371/journal.pone.0047046.tGenome Analyzer, yielding a total of four experiments (Table 1). A total of 668 and 4,331 reads from 454/Roche sequencing were analyzed for the non-PCR amplified and PCR amplified sample, respectively. These numbers include all reads overlapping at least 80 of the amino acids 10 to 93 of the HIV-1 protease and represent the coverage of this region, which hosts the mutations associated with resistance to protease inhibitors. Segments of the reads falling outside of this region were discarded. The length of the remaining segments is 232616 bases (mean 6 std) and 236618 bases for the two 454/Roche samples. Since we are dealing with a coding region, all insertions causing a frameshift were discarded. We did not detect any amino acid insertion or deletion. The Illumina experiments had a much higher throughput with more than one million reads mapped to the protease and local coverage of around 10,000 reads per base pair in the region further analyzed (Table 1). Reads from the 454/Roche platform are long enough to display the diversity of the viral pop.

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Author: DNA_ Alkylatingdna