The above conclusions are consistent with the investigation findings on Arabidopsis
The above conclusions are consistent together with the study findings on Arabidopsis thaliana and rice. We also discovered that exogenous BR spraying upregulates theanine synthesis genes, namely, TS, GS, and ADC and cold resistance-related genes, namely, CBF, ICE. It could be inferred that exogenous BR spraying elevated the theanine content material in tea leaves and improved cold resistance of tea plants. Theanine (L-Theanine) is usually a exclusive cost-free amino acid in tea and primary element of tea. Our findings are concordant towards the study final results of Li et al. (2018). The impact of exogenous BR spraying on the growth and development of tea leaves plus the signal transduction pathway of BR in tea leaves was revealed by transcriptome analysis. Our benefits showed that the tea leaves sprayed with BRs were considerably different, and also the upregulated genes were mainly connected to BR signal transduction, sucrose synthesis, chlorophyll synthesis, starch synthesis, flavonoid biosynthesis, cell division, theanine synthesis, and cold resistance. Furthermore, we also discovered that following spraying BR, the crucial genes for caffeine synthesis had been down-regulated. Our study lays the foundation for elucidating the molecular mechanism of the BR signal transduction pathway in tea leaves and its regulatory function around the growth and improvement of tea plants.Supplementary InformationThe on-line version contains supplementary material offered at doi. org/10.1186/s12864-021-08179-9. More file 1. Acknowledgements We thank LetPub (www.letpub.com) for its linguistic help during the preparation of this manuscript. Authors’ contributions QJ developed and performed the experiments, analyzed the data, drafted the paper. ZW ready figures and tables. YC, YL, and NT participated in the experiment and data evaluation. SL, JH and ZL conceived from the research,By way of KEGG Filovirus site enrichment and annotation, the UGP, SPS, GPI, PFP and EP genes involved in sucrose synthesis; the GSA, HEMD, POR, CHLH, and COA genes associated to ginseng chlorophyll synthesis; the AGPase,Jin et al. BMC Genomics(2022) 23:Web page 12 ofparticipated in coordination, information evaluation and interpretation, drafted and reviewed the manuscript. All the authors read and approved the final manuscript. Funding This function was financially supported by the National Natural Science Foundation of China (32172629, U19A2030, 31670689), Provincial Natural Science Foundation of Hunan (2020JJ4358), and Unique Project of Central Government Guiding Neighborhood Science and Technologies Improvement (2019XF5041). Availability of information and materials All of the data supporting our findings are contained inside the manuscript. All raw transcriptome information reported within this short article happen to be deposited within the Sequence Study Archive (SRA) under accession quantity PRJNA756445.9.ten. 11.12.DeclarationsEthics approval and Na+/H+ Exchanger (NHE) Inhibitor medchemexpress Consent to participate The tea trees utilised in this study came in the Chang’an Tea Teaching and Experimental Base of Hunan Agricultural University, Changsha City, Hunan Province, China, and no material license was needed. Our research is in complete compliance using the regulations with the method. This short article has obtained a license to use KEGG computer software from Kanehisa Labs [402]. Consent for publication Not applicable. Competing interests The authors have no conflict of interest to declare. Author information 1 Crucial Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China. two National Investigation Center of Engineering and Technology for Utiliz.