Ble in PMC 2018 January 05.Lin et al.PageExtended DataEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure 1. ZBP1 deficiency strongly delays and ameliorates skin inflammation in RIPK1E-KO mice.a, Photographs of mice using the indicated genotypes in the age of 4 weeks. Photos shown are representative of n60 RIPK1E-KO and n40 RIPK1E-KO Zbp1-/- mice. b, Photographs of mice using the indicated genotypes and age. Photos shown are representative of n4 RIPK1E-KO mice at the age of 5-7 weeks and n20 RIP1E-KO Zbp1-/mice at the age of 17-35 weeks.Nature. Author manuscript; offered in PMC 2018 January 05.Lin et al.Pagec, Table summarizing the macroscopically observed skin lesions and time of sacrifice of 21 aged RIPK1E-KO Zbp1-/- mice. d, Representative images of skin sections from RIPK1E-KO Zbp1-/- mice and their respective controls stained with H E (n18) or immunostained with the indicated antibodies (n4) at the age of 17-35 weeks. Nuclei stained with DAPI. Scale bars, 50 m. e, Representative photos of skin sections from 4-5 week old RIPK1E-KO (n6) and RIPK1E-KO Zbp1-/- (n3) and their respective control mice stained with TUNEL or immunostained with anti-CC3 antibodies. Nuclei stained with DAPI. Scale bars, 50 m. f, Microscopic quantification of CC3 and TUNEL good cells on skin sections from 4-5 week old mice with the indicated genotypes. Epi, Epidermis; Der, Dermis.Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; readily available in PMC 2018 January 05.Lin et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure two. ZBP1 is just not expected for keratinocyte necroptosis and skin inflammation in mice with epidermis-specific FADD deficiency.a, Representative photographs depicting the macroscopically observed phenotype of FADDE-KO (n10) and FADDE-KO Zbp1-/- (n=5) mice at the age of six days. b, Representative photos of skin sections from six day old mice with the indicated genotypes stained with H E (n=6) or immunostained together with the indicated antibodies (n=3). Nuclei stained with DAPI. Scale bars, 50 m.Nature. Author manuscript; readily available in PMC 2018 January 05.Eotaxin/CCL11 Protein custom synthesis Lin et al.CD160 Protein MedChemExpress Pagec, qRT-PCR analysis from the mRNA expression of your indicated cytokines and chemokines in total skin RNA from 6 day old mice using the indicated genotypes.Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure three.PMID:23927631 CRISPR/Cas9 mediated generation of Ripk1mRHIM and Mlkl-/-mice.a, Schematic depiction from the generation of Ripk1mRHIM/mRHIM mice indicating the sequence in the sgRNA plus the single stranded oligo utilised for mutating the RHIM domain that had been introduced by pronuclear injection into mouse zygotes along with the sequencing result of one of many two obtained founders.Nature. Author manuscript; accessible in PMC 2018 January 05.Lin et al.Pageb, Tiny intestinal sections from E18.five pups had been stained with H E or TUNEL or immunostained with anti-CC3 antibodies. Representative pictures shown (WT n=6 for H E, n=3 for TUNEL and anti-CC3; Ripk1mRHIM/mRHIM n=5 for H E and n=3 for TUNEL and anti-CC3; Ripk1-/- n=3 for H E, TUNEL and anti-CC3). Nuclei stained with DAPI. Scale bars, 50 . c, Microscopic quantification of CC3 and TUNEL good cells on gut sections from E18.5 pups with all the indicated genotypes. d, Diagram indicating the sgRNA target sequence (capital letters) employed to generate a mutation in exon 2 downstream of the ATG.
